Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) E. coli (ATCC 25922); ( b ) clinical isolate of imipenem-resistant E. coli with (red curves) and without (black curves) imipenem treatment; ( c ) signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( d ) r 654 of imipenem-resistant E. coli as a function of imipenem treatment time; ( e ) signal ratio of 724-cm −1 SERS peak ( r 724 ) of E. coli (ATCC 25922) as a function of imipenem treatment time; ( f ) r 724 of imipenem-resistant E. coli as a function of imipenem treatment time. Black and red curves represent the mean SERS spectra, while gray and light red curves represent their corresponding standard deviation.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Standard Deviation

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: After being treated by antibiotics for 0, 0.5, 1 and 2 hours, the bacteria were stained with PI (fluorescing red) and SYTO 9 (fluorescing green). BF: bright-field image; FL: fluorescence image. ( a ) S. aureus (ATCC 29213) treated with oxacillin; ( b ) E. coli (ATCC 25922) treated with imipenem; ( c ) percentage of dead bacteria ( f dead ): S. aureus (black columns) and E. coli (gray columns). f dead = N r /( N g + N r − N g + r ), where N r is the count of the red signatures, N g is the count of the green signatures, and N g + r is the count that the green and red signatures overlap. The scale bars represent 20 μ m.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques: Bacteria, Staining, Fluorescence

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a ) Signal ratio of 730-cm −1 SERS peak ( r 730 ) of S. aureus versus vancomycin treatment time at concentrations of 0.5 μ g/ml (light gray columns), 1 μ g/ml (gray columns), and 2 μ g/ml (black columns); ( b , c ) signal ratios of 654-cm −1 SERS peak ( r 654 ) and 724-cm −1 SERS peak ( r 724 ) of E. coli , respectively, versus imipenem treatment time at concentrations of 0.03 μ g/ml (light gray columns), 0.06 μ g/ml (gray columns), and 0.12 μ g/ml (black columns).

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques:

Journal: Scientific Reports

Article Title: Rapid bacterial antibiotic susceptibility test based on simple surface-enhanced Raman spectroscopic biomarkers

doi: 10.1038/srep23375

Figure Lengend Snippet: ( a – c ) Signal ratio of 654-cm −1 SERS peak ( r 654 ) of E. coli of inoculum densities of 10 6 , 10 7 and 10 8 CFU/ml, respectively, after being treated with different concentrations of imipenem for 2 hrs.

Article Snippet: Methicillin-susceptible Staphylococcus aureus (ATCC 29213) and Escherichia coli (ATCC 25922 and ATCC 35218) were obtained from American Type Culture Collection (ATCC).

Techniques:

Journal: Biochimica et biophysica acta. Biomembranes

Article Title: Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components.

doi: 10.1016/j.bbamem.2020.183291

Figure Lengend Snippet: Scheme 1. Representation of lipopolysaccharide structure in E. coli bacterial strains. Adapted from [Ebbensgaard 2018] and [Gronow 2001].

Article Snippet: We performed binding studies on sonicated bacterial cells for E. coli strains ATCC 25922, D21 and D21f2 (Figure 8 and Table 3).

Techniques:

Journal: Biochimica et biophysica acta. Biomembranes

Article Title: Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components.

doi: 10.1016/j.bbamem.2020.183291

Figure Lengend Snippet: Figure 5. Binding of DNS-PMAP23 to E. coli strains with different LPS structures. D21: purple; D21f2: orange. [DNS-PMAP23] = 10 μM.

Article Snippet: We performed binding studies on sonicated bacterial cells for E. coli strains ATCC 25922, D21 and D21f2 (Figure 8 and Table 3).

Techniques: Binding Assay

Journal: Biochimica et biophysica acta. Biomembranes

Article Title: Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components.

doi: 10.1016/j.bbamem.2020.183291

Figure Lengend Snippet: Figure 6. Binding of DNS-PMAP23 with E. coli strains lacking CL or PG and CL, compared to the WT strain. A) Fraction of DNS-PMAP-23 bound to different E. coli strains. [DNS-PMAP23] = 10μM. B) Zeta-potential measurements of different E. coli strains (1×107 CFU/mL) in the presence of increasing peptide concentrations. WT (K-12 MG1655): blue; BKT12: green; BKT29: red.

Article Snippet: We performed binding studies on sonicated bacterial cells for E. coli strains ATCC 25922, D21 and D21f2 (Figure 8 and Table 3).

Techniques: Binding Assay, Zeta Potential Analyzer

Journal: Biochimica et biophysica acta. Biomembranes

Article Title: Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components.

doi: 10.1016/j.bbamem.2020.183291

Figure Lengend Snippet: Figure 7. Content of CL, PG and PA in different E. coli strains. The lipid contents are reported after normalization to the WT strain.

Article Snippet: We performed binding studies on sonicated bacterial cells for E. coli strains ATCC 25922, D21 and D21f2 (Figure 8 and Table 3).

Techniques:

Journal: Biochimica et biophysica acta. Biomembranes

Article Title: Binding of an antimicrobial peptide to bacterial cells: Interaction with different species, strains and cellular components.

doi: 10.1016/j.bbamem.2020.183291

Figure Lengend Snippet: Figure 8. Binding of DNS-PMAP23 to live and dead E. coli cells. ATCC 25922: blue; D21: green; D21f2: red. [DNS-PMAP23] = 10μM. Filled symbols and solid lines correspond to live bacteria, while empty symbols and dashed lines correspond to dead cells. Duplicate measurements are reported with different symbols.

Article Snippet: We performed binding studies on sonicated bacterial cells for E. coli strains ATCC 25922, D21 and D21f2 (Figure 8 and Table 3).

Techniques: Binding Assay, Bacteria

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Screening lead compounds for antibacterial activity against Gram-positive and Gram-negative bacteria. Two-fold dilutions of six lead compounds (labelled 1–6) were applied in 6 μL volumes to the surface of a soft agar overlay inoculated with Bacillus subtilis , Staphylococcus epidermidis , Escherichia coli or Pseudomonas fluorescens . The LB agar plates were exposed to light at 365 nm for 5 min and then incubated for 24 h at 37°C prior to imaging. Controls without light activation or with application of DMSO are shown in .

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Activity Assay, Bacteria, Incubation, Imaging, Activation Assay

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Effect of Compound 2 on bacterial growth. Escherichia coli (A) , B. subtilis (B) and S. epidermidis (C) were cultivated in LB broth at 37°C in 96-well plates in a plate reader with continuous shaking. Growth was monitored at OD 600nm in samples exposed to light at 365 nm for 5 min (filled symbols) or without light treatment (open symbols). Samples contained 2 μM Compound 2 or 0.2% DMSO indicated by circles or squares, respectively.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques:

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Real-time monitoring of bacterial membrane integrity. The BacLight assay of membrane integrity following photoactivation of Compound 2 . S. epidermidis (A) , B. subtilis (B) , P. fluorescens (C) and E. coli (D) in mid-log phase of growth were stained with PI (magenta) and SYTO 9 (yellow) and imaged by confocal microscopy without light activation (−) or 10 min after photoactivation with the 405 nm laser (+). The laser was applied at 30% power for 1 min. The bar represents 3 μm.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Membrane, BacLight Assay, Staining, Confocal Microscopy, Activation Assay

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Role of the E. coli outer membrane in protecting against Compound 2 toxicity. (A) The viability of E. coli O + , O − and Δ rfaC cells was evaluated as in with exposure to 2 μM Compound 2 with or without light activation. (B) PI assay of E. coli strains to monitor loss of membrane integrity. (C) Real-time imaging of E. coli strains stained with PI (magenta) and SYTO 9 (yellow) by confocal microscopy. Images taken prior to light exposure (−) and 10 min after photoactivation (+) as indicated. The bar represents 3 μm.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Membrane, Activation Assay, Imaging, Staining, Confocal Microscopy

Journal: Frontiers in Microbiology

Article Title: The antibacterial activity of a photoactivatable diarylacetylene against Gram-positive bacteria

doi: 10.3389/fmicb.2023.1243818

Figure Lengend Snippet: Susceptibility of E. coli strains deficient in oxidative damage and tolerance pathways. The viability of bacteria exposed to 2 μM Compound 2 after photoactivation is shown. Serial dilutions of bacteria were applied to LB agar plates and colonies counted to determine CFU/ml. A representative image of each strain is shown (A) and the CFU/ml (B) represents the mean and standard error of three independent experiments.

Article Snippet: Bacillus subtilis 168 (ATCC 23857), Staphylococcus epidermidis (ATCC 12228), Pseudomonas fluorescens (ATCC 13525) and Escherichia coli FDA strain Seattle 1946 (ATCC 25922) were obtained from the American Type Culture Collection.

Techniques: Bacteria

Journal: International Journal of Molecular Sciences

Article Title: Green Synthesis of Silver Nanoparticles with Antibacterial, Anti-Inflammatory, and Antioxidant Activity Using Convolvulus arvensis

doi: 10.3390/ijms27031210

Figure Lengend Snippet: Scanning Electron Microscopy (SEM) images showing the morphological effects of silver nanoparticles synthesized from C. arvensis. ( A – C ) demonstrate the effect on Gram-positive S. aureus (MRSA and ST), E-H demonstrate the effect on Gram-negative E. coli ESBL and ST) bacterial strains. described above, and the cells exhibited a division-related septum, broadening, and cytoplasm leakage. Scanning electron microscope images showed that the untreated control groups ( A , C , E , G ), bacterial cells exhibited normal morphology with intact cell walls and smooth surfaces. In contrast, treated cells ( B , D , F , H ) showed significant morphological changes: S. aureus cells displayed shrinkage and deformations in their spherical shape, while E. coli cells exhibited marked structural disintegration and severe membrane damage. These observations confirm the destructive effects of silver nanoparticles on bacterial cell integrity. Most of the cells were damaged and perforated, which changed the cell structure and shape, and it was noted that some of them were empty. In addition, most cells appeared to stick together compared to untreated cells. As is clear in .

Article Snippet: Four bacterial strains were used in this study: one standard Gram-positive strain ( Staphylococcus aureus ATCC 29213 ), one standard Gram-negative strain ( Escherichia coli ATCC 25922 ), and two clinical isolates (SP1 and SP2) obtained from patient samples.

Techniques: Electron Microscopy, Synthesized, Microscopy, Control, Membrane

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Structure of pantothenic acid and N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Synthetic route to new N-substituted pantothenamides

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques:

Journal:

Article Title: Geminal dialkyl derivatives of N -substituted pantothenamides: Synthesis and antibacterial activity

doi: 10.1016/j.bmc.2011.02.053

Figure Lengend Snippet: Antibacterial activity of N -substituted pantothenamides 9a–9m

Article Snippet: A preliminary screen of these pantothenamides against other strains ( Acinetobacter baumannii ATCC 19606, Klebsiella penumoniae ATCC 13883, Escherichia coli ATCC 25922 and 11775, and Bacillus subtilis ATCC 6051 and 6633) using the agar diffusion method suggests no antimicrobial activity (inhibition zone <1.1 cm for a 1 cm disc impregnated with the desired compound). table ft1 table-wrap mode="anchored" t5 caption a7 MIC (μM) a S. aureus b MRSA c Open in a separate window 9a 7 ± 6 7± 3 Open in a separate window 9b 13 ± 7 13± 2 Open in a separate window 9c 101 ± 45 51 ± 15 Open in a separate window 9d 374 ± 187 374 ± 153 Open in a separate window 9e >715 >715 Open in a separate window 9f 3.2 ± 0.8 3.2 ± 0.9 Open in a separate window 9g >703 >703 Open in a separate window 9h >744 >744 Open in a separate window 9i 376 ± 217 376 ± 109 Open in a separate window 9j 1 ± 0.9 1 ± 0.7 Open in a separate window 9k(N5-pan) 7 ± 2 7 ± 2 Open in a separate window 9l (N9-pan) 0.4 ± 0.2 0.4 ± 0.2 Open in a separate window 9m 24 ± 14 24 ± 14 Open in a separate window a Minimum inhibitory concentrations (MICs) were determined using the following concentrations: 0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL.

Techniques: Activity Assay